Development Optimization of Anthelmintic Activity of Herbal Formulation

Abstract

Various Asian traditional medical systems have reported the use of Azadirachta indica leaves and Trichosanthes dioica seeds as herbal remedies for anthelmintic purposes in ethnopharmacology. The present study aimed to develop a herbal formulation containing A. indica leaves and T. dioica seeds and to evaluate its anthelmintic activity in order to determine the optimal dose for maximum efficacy.

For tablet formulation, preformulation studies were conducted. Experimental investigations were carried out on nematodes and Ascaridia galli to assess and compare the anthelmintic efficacy of three formulations (F1, F2, and F3). Albendazole was used as the standard reference drug. For each formulation and the standard, the time required for worm paralysis and death (lethal time) was recorded.

The results demonstrated that A. was significantly paralyzed and killed by all tested formulations. Among them, formulation F1, containing a 1:1 ratio of both plant extracts, showed the highest anthelmintic activity by exhibiting the shortest paralysis and lethal times.